Application of Glow Discharge Aes for Investigation of Metal Ions and Water in Biology and Medicine

TL;DR

This paper explores the use of glow discharge atomic emission spectroscopy (AES) for rapid analysis of metal ions and water in biological samples, revealing structural changes and potential disease markers.

Contribution

It introduces a novel application of GD AES for analyzing hydration energy, metal-macromolecule complexes, and disease-related metal content in biological solutions.

Findings

Hydration energy increases by 0.65 kcal/mole in hydrated water molecules.

Method enables analysis of high organic content solutions, aiding health risk assessment.

Altered copper levels in blood serum correlate with lymphogranulomatosis.

Abstract

AES VHF inductively coupled plasmatron may be applied to wide range of studies. It enables rapid microanalysis of various solutions including biological objects and peripheral blood serum. In addition, it may be used for investigation of water desorption from solid bodies and for determination of energetic metal-macromolecule complexes. Study of hydration energy and hydration number by kinetic curves of water glow discharge atomic spectral analysis of hydrogen (GD EAS analysis of hydrogen) desorption from Na-DNA humidified fibers allowed to reveal that structural and conformational changes in activation energy of hydrated water molecules increases by 0.65kcal/Mole of water. The developed method of analysis of elements in solutions containing high concentrations of organic materials allows systematic study of practically healthy persons and reveals risk factors for several diseases. Microelemental content of blood serum fractions showed that amount of not bounded with ceruloplasmin copper was three times more at limphogranulomatose disease than that in healthy persons.