Glow Discharge AES: Methodological Peculiarities of Pulse Element Analysis and Flash Desorption

TL;DR

This paper discusses various Glow Discharge AES techniques, emphasizing parameters like VHF-field power, inert gas pressure, and additives, and introduces a high-sensitivity flash desorption method for biological samples.

Contribution

It presents a novel high-sensitivity flash desorption technique for biological samples and analyzes the influence of key parameters on Glow Discharge AES performance.

Findings

Electron temperature of helium is approximately 15000 K.

Detection limits depend on background signal and standard deviation.

The flash desorption method enables water desorption rate investigation from biological samples.

Abstract

Different techniques of Glow Discharge AES are described in this paper. The most important parameters at such investigations are: the power of VHF-field, pressure of the inert gas and concentration of the easily ionizable additive, e.g. NaCl. The influences of these parameters were studied It is proposed a high sensitive flash desorption method, which enables investigation of the water desorption rate from humidified samples of biological origin, namely DNA and chromatin. The ways of minimizing of detection limit are considered as the most important characteristics of an analytical device. The concentration of any measured element is detectable if it correlates to the signal equal to tripled standard deviation of the results of background measurement. Electron temperature of the Helium has been evaluated by absorption rate at two lines of helium \lambda=353.828nm; \lambda'=344.759nm that was equal to T_c ~ 15000 K.