Exploring nervous system transcriptomes during embryogenesis and metamorphosis in Xenopus tropicalis using EST analysis

TL;DR

This study generated a comprehensive EST-based transcriptome resource for Xenopus tropicalis's nervous system during embryogenesis and metamorphosis, aiding functional genomics and genetic marker development.

Contribution

It provides the first large-scale EST dataset for X. tropicalis nervous system development, including gene annotation, expression profiles, and genetic markers.

Findings

Identified 9,693 transcripts from 6,000 genes.

Developed 225 polymorphic microsatellites.

Defined stage-specific gene expression profiles.

Abstract

Xenopus tropicalis is an anuran amphibian species used as model in vertebrate comparative genomics. It provides the same advantages as Xenopus laevis but is diploid and has a smaller genome of 1.7 Gbp. Therefore X. tropicalis is more amenable to systematic transcriptome surveys. We initiated a large-scale partial cDNA sequencing project to provide a functional genomics resource on genes expressed in the nervous system during early embryogenesis and metamorphosis in X. tropicalis. A gene index was defined and analysed after the collection of over 48,785 high quality sequences. Partial cDNA sequences were obtained from an embryonic head and retina library (30,272 sequences) and from a metamorphic brain and spinal cord library (27,602 sequences). These ESTs are estimated to represent 9,693 transcripts derived from an estimated 6,000 genes. An estimated 46% of these cDNA sequences contain their start codon. Further annotation included Gene Ontology functional classification, InterPro domain analysis, alternative splicing and non-coding RNA identification. Gene expression profiles were derived from EST counts and used to define transcripts specific to metamorphic stages of development. Moreover, these ESTs allowed identification of a set of 225 polymorphic microsatellites that can be used as genetic markers. These cDNA sequences permit in silico cloning of numerous genes and will facilitate studies aimed at deciphering the roles of cognate genes expressed in the nervous system during neural development and metamorphosis. The genomic resources developed to study X. tropicalis biology will accelerate exploration of amphibian physiology and genetics.