Temperature micro-Raman study of lysozyme crystals

TL;DR

This study uses micro-Raman spectroscopy to investigate lysozyme crystals at temperatures below room temperature, revealing anomalous shifts in Raman bands near 285K that relate to structural changes.

Contribution

First micro-Raman analysis of lysozyme crystals at sub-room temperatures, identifying temperature-dependent spectral anomalies linked to structural transitions.

Findings

Detected anomalous Raman band shifts below 285K

Observed temperature dependence of lysozyme crystal structure

Identified potential structural transition near 285K

Abstract

We have performed for the first time micro-Raman studies of lysozyme crystals for the temperatures lower than the room one (in the range 282-300K). Anomalous temperature dependence of the positions of Raman scattering bands has been detected and discussed, which corresponds to the crystalline lysozyme structure in the vicinity of T=285K.