Characterizing synaptic conductance fluctuations in cortical neurons and their influence on spike generation

TL;DR

This paper reviews methods to analyze synaptic conductance fluctuations in cortical neurons, highlighting their impact on spike generation, and introduces new techniques for characterizing synaptic activity from intracellular recordings.

Contribution

It presents a stochastic conductance model and analytical methods to extract synaptic parameters from Vm data, advancing understanding of synaptic influence on neuronal firing.

Findings

Inhibition plays a key role in cortical neuron dynamics.

Conductance variance significantly influences spike timing.

Methods effectively analyze in vivo and in vitro synaptic activity.

Abstract

Cortical neurons are subject to sustained and irregular synaptic activity which causes important fluctuations of the membrane potential (Vm). We review here different methods to characterize this activity and its impact on spike generation. The simplified, fluctuating point-conductance model of synaptic activity provides the starting point of a variety of methods for the analysis of intracellular Vm recordings. In this model, the synaptic excitatory and inhibitory conductances are described by Gaussian-distributed stochastic variables, or colored conductance noise. The matching of experimentally recorded Vm distributions to an invertible theoretical expression derived from the model allows the extraction of parameters characterizing the synaptic conductance distributions. This analysis can be complemented by the matching of experimental Vm power spectral densities (PSDs) to a theoretical template, even though the unexpected scaling properties of experimental PSDs limit the precision of this latter approach. Building on this stochastic characterization of synaptic activity, we also propose methods to qualitatively and quantitatively evaluate spike-triggered averages of synaptic time-courses preceding spikes. This analysis points to an essential role for synaptic conductance variance in determining spike times. The presented methods are evaluated using controlled conductance injection in cortical neurons in vitro with the dynamic-clamp technique. We review their applications to the analysis of in vivo intracellular recordings in cat association cortex, which suggest a predominant role for inhibition in determining both sub- and supra-threshold dynamics of cortical neurons embedded in active networks.