Influence of experimental context on the development of anhedonia in male mice imposed to chronic social stress

TL;DR

This study investigates how different experimental contexts affect the development of anhedonia in male mice subjected to chronic social stress, revealing that stress reduces sucrose intake and that context influences this behavior.

Contribution

It demonstrates that the experimental setting significantly impacts anhedonia development in stressed mice, highlighting the importance of context in depression models.

Findings

Stressed mice show reduced sucrose intake compared to controls.

Context influences the degree of anhedonia observed in mice.

Chronic social stress leads to decreased liquid consumption in mice.

Abstract

Anhedonia is one of the key symptoms of depression in humans. Consumption of 1% sucrose solution supplemented with 0.2% vanillin was studied in two experimental contexts in male mice living under chronic social stress induced by daily experience of defeats in agonistic interactions and leading to development of depression. In the first experiment, vanillin sucrose solution was made available as an option of water during 10 days to mice living in group home cages. Then the mice were subjected to social defeat stress and during stress exposure they were provided with both vanillin sucrose solution and water using a free two bottles choice paradigm. In the other experiment, vanillin sucrose solution were first offered to mice after 8 days of exposure to social defeat stress. Males familiar with vanillin sucrose solution showed vanillin sucrose preference while experiencing defeat stress: consumption of vanillin sucrose solution was about 70% of total liquid consumption. However, the consumption of vanillin sucrose solution per gram of body weight in mice imposed to social stress during 20 days was significantly lower than in control males. In the second experiment, males after 8 days of social defeat stress were found to consume significantly less vanillin sucrose solution as compared with control males. On average during two weeks of measurements, vanillin sucrose solution intake was less than 20% of total liquid consumption in males with symptoms of depression and anxiety. Consumption per gram of body weight also appeared to be significantly lower than in control group. Influence of the experimental context on the development of anhedonia, which was measured by the reduction in sucrose solution intake by chronically stressed male mice, has been discussed.